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. 2020 Aug 3;117(33):19963–19969. doi: 10.1073/pnas.2004138117

Fig. 1.

Fig. 1.

Equilibrium denaturation experiments. (A) GdnHCl-induced equilibrium denaturation of WT P1-P2 (open diamonds) and pP1-P2 (black circles) monitored by fluorescence. The change in the intrinsic fluorescence of the tryptophan residue vs. GdnHCl concentrations is consistent with a two-state transition. No detectable change could be observed in the case of WT P1-P2. (B) GdnHCl-induced equilibrium denaturation of WT P1-P2 (open diamonds) and pP1-P2 (black circles) monitored by CD. As discussed in the text, the denaturation obtained by CD experiments does not fit well to a two-state transition, revealing an additional complexity. (C) GdnHCl-induced equilibrium denaturation of pP1-P2 (black circles), pPDZ1 (empty circles), and PDZ2 (gray circles) monitored by CD. The curves obtained for pPDZ1 and PDZ2 are consistent with a two-state transition. The pP1-P2 denaturation curve was satisfactorily fitted by the sum of the individual curves obtained for pPDZ1 and PDZ2 in isolation.