Fig. 6.

Inhibition of de novo synthesis of Rev in virus-infected cells abolishes SIR. (a) Integration events per cell were estimated following treatment with cycloheximide as described in Methods and then treated cells were 1st-INF with INm HIV-1 (m.o.i.=1) and 2nd-INF with the indicated wt viruses (m.o.i.=1 or 10). (b) Same as in (a), but cells were treated with actinomycin D. (c) Western blot analysis of INm HIV-1-infected cells incubated with the indicated inhibitors. (d) Same as in (a), but in the presence of AZT. (e) Effects of treatment with cycloheximide or with actinomycin D on integration levels following a single infection with wt HIV-1. All experimental details are as described in Methods.