Skip to main content
. Author manuscript; available in PMC: 2020 Aug 24.
Published in final edited form as: Anal Chem. 2019 Mar 14;91(7):4266–4290. doi: 10.1021/acs.analchem.9b00807

Figure 6.

Figure 6.

Highlights of the application of ambient ionization MS for the analysis of live bacterial colonies. The left panel shows LAESI-MS images of live bacterial colonies at different depths, created by imaging, subsequently sputtering the surface, and imaging again. Antibiotic susceptibility of E. coli and B. subtilis were evaluated in the same LAESI-MS image. Reproduced from Molecular Imaging of Growth, Metabolism, and Antibiotic Inhibition in Bacterial Colonies by Laser Ablation Electrospray Ionization Mass Spectrometry, Li, H.; Balan, P.; Vertes, A. Angew. Chem. Int. Ed. Engl., Vol. 55, Issue 48 (ref 166). Copyright 2016 Wiley. The right panel shows the use of DESI-MS imaging to monitor catalytic activity in bacterial colonies. The catalytic reaction monitored was the asymmetric addition of ammonia to cinnamic acid by the PAL enzyme. DESI-MS ion images show the distribution of a membrane lipid (C18:1) to locate colonies, and the distribution of the reaction product (2a). Reprinted from Yan, C.; Parmeggiani, F.; Jones, E. A.; Claude, E.; Hussain, S. A.; Turner, N. J.; Flitsch, S. L.; Barran, P. E. J. Am. Chem. Soc. 2017, 139, 1408–1411 (ref 167). Copyright 2017 American Chemical Society.