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. 2020 Aug 24;11:363. doi: 10.1186/s13287-020-01862-w

Table 1.

Primers used for RT-PCR

Gene Primer sequence Accession number Amplicon (bp)
18S

5′-GTA ACC CGT TGA ACC CCA TT-3′

5′-CCA TCC AAT CGG TAG TAG CG-3′

NR_003286.2 151
SOX9

5′-GTA CCC GCA CTT GCA CAA C-3′

5′- TCT CGC TCT CGT TCA GAA GTC-3′

NM_000346.3 74
Collagen 2A

5′- GGGAGTAATGCAAGGACCA-3′

5′- ATCATCACCAGGCTTTCCAG-3′

NM_001844.4 175
Aggrecan

5′-GCC TGC GCT CCA ATG ACT-3′

5′-ATG GAA CAC GAT GCC TTT CAC-3′

NM_013227.3 104
Collagen A1

5′-CCC CTG GAA AGA ATG GAG ATG-3′

5′-TCC AAA CCA CTG AAA CCT CTG-3′

NM_000088.3 148
MMP13

5′-TCA CCA ATT CCT GGG AAG TCT-3′

5′-TCA GGA AAC CAG GTC TGG AG-3′

NM_002427.3 95
Collagen 10

5′-ACG CTG AAC GAT ACC AAA TG-3′

5′-TGC TAT ACC TTT ACT CTT TAT GGT GTA-3′

NM_000493.3 101
p53

5′-GGCCCACTTCACCGTACTAA-3′

5′-GTGGTTTCAAGGCCAGATGT-3′

NM_000546 156
BAX

5′-TTTGCTTCAGGGTTTCATCC-3′

5′-CAGTTGAAGTTGCCGTCAGA-3′

NM_001291428.1 246