Table 1.
Gene | Primer sequence | Accession number | Amplicon (bp) |
---|---|---|---|
18S |
5′-GTA ACC CGT TGA ACC CCA TT-3′ 5′-CCA TCC AAT CGG TAG TAG CG-3′ |
NR_003286.2 | 151 |
SOX9 |
5′-GTA CCC GCA CTT GCA CAA C-3′ 5′- TCT CGC TCT CGT TCA GAA GTC-3′ |
NM_000346.3 | 74 |
Collagen 2A |
5′- GGGAGTAATGCAAGGACCA-3′ 5′- ATCATCACCAGGCTTTCCAG-3′ |
NM_001844.4 | 175 |
Aggrecan |
5′-GCC TGC GCT CCA ATG ACT-3′ 5′-ATG GAA CAC GAT GCC TTT CAC-3′ |
NM_013227.3 | 104 |
Collagen A1 |
5′-CCC CTG GAA AGA ATG GAG ATG-3′ 5′-TCC AAA CCA CTG AAA CCT CTG-3′ |
NM_000088.3 | 148 |
MMP13 |
5′-TCA CCA ATT CCT GGG AAG TCT-3′ 5′-TCA GGA AAC CAG GTC TGG AG-3′ |
NM_002427.3 | 95 |
Collagen 10 |
5′-ACG CTG AAC GAT ACC AAA TG-3′ 5′-TGC TAT ACC TTT ACT CTT TAT GGT GTA-3′ |
NM_000493.3 | 101 |
p53 |
5′-GGCCCACTTCACCGTACTAA-3′ 5′-GTGGTTTCAAGGCCAGATGT-3′ |
NM_000546 | 156 |
BAX |
5′-TTTGCTTCAGGGTTTCATCC-3′ 5′-CAGTTGAAGTTGCCGTCAGA-3′ |
NM_001291428.1 | 246 |