Fig. 3.

The regulation of actin assembly and disassembly by Thor gelsolins followed by TIRF microscopy. Time course of the (A) assembly and (B) disassembly of 1.5 μM actin in the presence of various concentrations of Thor gelsolins. (Scale bar, 20 μm.) Titrations of ProGel and 1DGelX in the assembly assay can be found in SI Appendix, Fig. S5, and comparison of 2DGel, 2DGel2, and 2DGel3 in the disassembly assay are found in SI Appendix, Fig. S6. Movies of the assembly/disassembly of Thor gelsolins are found in Movies S1–S5. ProGel and 1DGelX assays were carried out in 1 mM EGTA. The 2DGel assembly assays were in 0.3 mM CaCl₂ or 1 mM EGTA, and the disassembly assay in 0.3 mM CaCl₂.