Table 1.
Primers for Real-Time Polymerase Chain Reaction.
| Genes | Forward | Reverse |
|---|---|---|
| TF | GGAGGAGCCGCCATTTACAAA | AAACTGCTGAATTACTGGCTGT |
| ICAM-1 | GTGATGCTCAGGTATCCATCCA | CACAGTTCTCAAAGCACAGCG |
|
CCL2
CXCL10 |
TAAAAACCTGGATCGGAACCAAA CCAAGTGCTGCCGTCATTTTC |
GCATTAGCTTCAGATTTACGGGT GGCTCGCAGGGATGATTTCAA |
| TLR4 | GCCTTTCAGGGAATTAAGCTCC | GATCAACCGATGGACGTGTAAA |
| IL-1β | TTCAGGCAGGCAGTATCACTC | GAAGGTCCACGGGAAAGACAC |
| IL-6 | AGCCAGAGTCCTTCAGA | GGTCCTTAGCCACTCCT |
| TNF-α | CCCTCACACTCAGATCATCTTCT | TGCTACGACGTGGGCTACAG |
| INS | CAATCATAGACCATCAGCAAGC | AGAAACCACGTTCCCCAC |
| PDX1 | CCCGAGCTTCTGAAAACTTTG | CTTTTCATTGTCCTCAGTTGGG |
| MAFA | AGGAGGAGGTCATCCGACTG | CTTCTCGCTCTCCAGAATGTG |
| NKX6.1 | CGCTTGGCCTATTCTCTGGG | CTGCGTGCTTCTTTCTCCA |
| GLUT2 | TGTGCTGCTGGATAAATTCGCCTG | AACCATGAACCAAGGGATTGGACC |
| GAPDH | CATGGCCTTCCGTGTTCCTA | GCGGCACGTCAGATCCA |
TF: tissue factor; ICAM-1: intercellular adhesion molecule; CCL2: chemokine (C-C motif) ligand 2; CXCL10: C-X-C motif chemokine 10; TLR4: Toll-like receptor 4; IL-1β: interleukin-1β; IL-6: interleukin-6; TNF-α: tumor necrosis factor-α.