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. 2020 Feb 11;29:0963689719897061. doi: 10.1177/0963689719897061

Figure 4.

Figure 4.

MTDH is a target of miR-497-5p. (A) 3’-UTR luciferase reporter assays. SKOV-3 and TOV-112D cells were co-transfected with miR-497-5p or miR-NC and MTDH 3’-UTR luciferase reporter construct. Firefly luciferase activity was normalized to Renilla luciferase activity. (B) Relative level of MTDH mRNA in SKOV-3 and TOV-112D cells was determined by qRT-PCR after miR-497-5p overexpression. (C) MTDH protein expression in SKOV-3 and TOV-112D cells was determined by western blotting after miR-497-5p overexpression. *p < 0.05 vs. untransfected cells; ^p < 0.05 vs. miR-NC; n = 3.