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. 2020 Feb 5;29:0963689719885077. doi: 10.1177/0963689719885077

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Umbilical cord-derived MSCs inhibit the proliferation of leukemic tumor cells through cell-to-cell contact. Tumor cells at 0.5 × 10⁵ cells/well of 96-well plates were cultured in the presence or absence of UC-MSC at various ratios for 3 days. Cell viability and proliferation were assessed by MTS assay (Panel a) and ³H-TdR incorporation (Panel b) during the final 4 and 18 h of culture, respectively. The results represent the mean of at least four experiments ±SD. Panel (c) shows the tumor cells were cultured alone (Tumor cell), in direct contact with UC-MSC (Tumor+UC-MSCs), with UC-MSCs separated by Transwell (Transwell+Tumor) and in supernatant aspirated from UC-MSCs culture (SN+Tumor). Data show results from more than three independent experiments with mean ±SD. [*] statistically significant at p<0.05.