Figure 3.

Protein expression validation of selected genes after co-culture with UC-MSCs. One million leukemic tumor cells were culture in presence or absence of UC-MSC at 1:01 ratio for 72 h. After 3 days cell were lysed and proteins were loaded onto SDS-PAGE gel and separating by electrophoresis. Then proteins were transferred onto PVDF membrane and incubated with primary antibodies and then secondary antibody for overnight and 1 h, respectively. All western blots were performed with two independent biological samples and repeated at least thrice. The representative data are shown.