Table 4.
mRNA Expression of Selected Genes after Co-cultured with UC-MSCs were Validated by RT-qPCR in BV173 and HL-60 Cell Lines.
| Genes | HL-60 Cell line | BV173 Cell line | ||
|---|---|---|---|---|
| Microarray FC | RT-qPCR FC | Microarray FC | RT-qPCR FC | |
| MDM2 | -9.355383 | -21.93 | -4.118405 | -17.85 |
| CCNE2 | -5.861723 | -12.04 | -2.821386 | -2.93 |
| MYL9 | -4.603044 | -5.29 | 530.4346 | 1351.17 |
| TGFβI | -365.5724 | -858.09 | 25509.12 | 30573.62 |
| CDKN1A | 3.77062 | 4.80 | 5.14529 | 12.99 |
| CDKN2A | 2.224852 | 3.61 | 3.622785 | 3.86 |
For microarray result validation, the extracted RNA from leukemic tumor cells in presence or absence of UC-MSCs were converted to cDNA and after designing primer pairs for selected genes, the qRT-PCR reaction was run using Light Cycler 480 DNA SYBR Green I Master by Roche. Then, the relative fold change in RNA expression was analyzed using the 2−ΔΔ C T method. The RT-qPCR results show that the mRNA expression level in HL-60 and BV173 cells in the presence of UC-MSCs showed similar results with the microarray (n=3/ triplicate).