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. 2020 Aug 24;11:366. doi: 10.1186/s13287-020-01883-5

Fig. 5.

Fig. 5

Effects on photoreceptors at middle developmental stage after COCO supplement. a Percentage mRNA expression of NRL, RHO, and OPN1MW transcripts in D90 organoids analyzed by qPCR in COCO supplement and control. b Percentage mRNA expression of OPN1SW transcripts in D45, D60, and D90 organoids. c, d Immunocytochemical analysis and quantification for NRL. Data are expressed as mean ± SEM. Scale bar, 50 μm. e The proposed effect of COCO at early developmental stage intervention in 3D retinalization. *P < 0.05