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. 2020 Aug 24;25:40. doi: 10.1186/s11658-020-00232-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Analysis of expression of stress-induced molecules. a Western analysis of the hearts of 3-month old male L3 and L9 mice. b Western analysis of the hearts of 4-week old male L9 mice. c Quantitative RT-PCR analysis of G6pd and p62 in the hearts of 3-month old male L9 mice. The results are presented as the means ± SD. Significance was assessed with unpaired t-test (n = 4 ~ 9; **p < 0.01 vs. NTG). d Western analysis of the hearts of 3-month-old male L9 mice. The white and black arrowheads respectively indicate LC3-I and LC3-II. An arrow indicates Nrf2. The right panels show relative densitometric analysis of the western blot of LC3. The results are presented as the means ± SD. Significance was assessed with unpaired t-test (n = 4; *p < 0.05 vs. NTG). a, b and d: Whole cell extracts were examined with the indicated antibodies. e Quantitative RT-PCR analysis of Nqo1 and Gsta1 in the hearts of 3-month old male L9 mice. The results are presented as the means ± SD. Significance was assessed with unpaired t-test (n = 4 ~ 6; *p < 0.05 vs. NTG). a–e Similar results were obtained in at least two independent experiments