Table 2.

De novo transcriptome assembly statistics with Trinity program for 17 RNA samples of four rye inbred lines

Sample	All transcript contigs	Only longest isoform per ‘GENE’
Total trinity ‘genes’	150,758	150,758
Total trinity transcripts	218,935	150,758
N50	1366	1366
Maximum contig length	16,024	16,024
Minimum contig length	201	201
Median contig length	500	401
Average contig length	839.29	703.25
Total assembled bases	183,750,947	106,021,123