Fig. 5.

Direct intratracheal instillation of ceramide induces phosphorylation of JAK2 and STAT3 in vivo. BALB/c mice were treated with AG490 at 15 mg/kg of body weight or Stattic at 5 mg/kg of body weight. The mice were then exposed to 5 mg/kg of C6-ceramide by intratracheal instillation. (a) JAK2 and p-JAK2 expression was examined by Western blotting, and relative quantification of the p-JAK2/JAK2 expression was determined by densitometric analysis of the blots. (b) STAT3 and p-STAT3 expression was examined by Western blotting, and relative quantification of the p-STAT3/STAT3 expression was determined by densitometric analysis of the blots. n = 6 per group. ^#P < 0.05, ^##P < 0.01 vs the control group; ^∗P < 0.05, ^**P < 0.05 vs the C6-ceramide-stimulated group