KEY REAGENTS AND RESOURCES
| Kits, Recombinant proteins and Chemicals | Source | Catalog # |
|---|---|---|
| EasySep Human CD3 Positive Selection Kit | Stem Cell Technologies, Vancouver, BC, Canada | 18051 |
| Qiagen RNA Extraction Kit | Qiagen, Hilden, Germany | 74104 |
| Human lung mRNA | Thermo Fisher Scientific, Waltham, MA, USA | AM7968 |
| One-Step RT -PCR kit | Qiagen, Hilden, Germany | 210210 |
| MPER mammalian protein extraction reagent | Thermo Fisher Scientific, Waltham, MA, USA | 78501 |
| Complete protease inhibitor cocktail tablets | Roche Diagnostics GmbH, Mannheim, Germany | 1186153001 |
| Ceramic beads | MP Biologicals, Irvine, CA, USA | 6913100 |
| Fast-prep homogenizer | MP Biologicals, Irvine, CA, USA | SKU 116005500 |
| BCA reagent | Thermo Fisher Scientific, Waltham, MA, USA | 23225 |
| S1(RBD)-mFcgamma2a chimeric antibody | ACRO Biosystems, Newark, DE, USA | S1N-C5257 |
| Rat anti-mouse lgG2a-magnetic microbeads | Miltenyi Biotec, Berdisch Gladback, Germany | 130-047-202 |
| MACS magnetic columns | Miltenyi Biotec, Berdisch Gladback, Germany | 130-042-401 |
| SARS-CoV-2 S protein, His Tag, Super stable trimer | ACRO Biosystems, Newark, DE, USA | SPN-C52H9 |
| SARS-CoV-2 N protein, His Tag | ACRO Biosystems, Newark, DE, USA | NUN-C51H9 |
| Bovine serum albumin (BSA) | Sigma-Aldrich, St. Louis, MO, USA | A7030-100g |
| SuperSignal™ West Pico PLUS Chemiluminescent Substrate | Thermo Fisher Scientific, Waltham, MA, USA | 34579 |
| NuPAGE™ 4 to 12%, Bis-Tris, 1.5 mm, Mini Protein Gel, 10-well | Thermo Fisher Scientific, Waltham, MA, USA | NP0335PK2 |
| Human lung total protein | Zyagen, San Diego, CA, USA | HT -601 |
| iBIot™ 2 Gel Transfer Device | Thermo Fisher Scientific, Waltham, MA, USA | IB21001 |
| Vectashield containing DAPI | Vector Laboratories, Burlingame, CA, USA | H-1200-10 |
| Tissue fixation/permeabilization buffer | Thermo Flsher Scientific, Waltham, MA, USA | 00-8333-56 |
| microplate reader | BioTek Instruments, Winooski, VT, USA | |
| Fluorchem E imaging system | ProteinSimple, San Jose, CA, USA | |
| RNA-STAT -60 extraction reagent | Tel-Test, Inv., Friendswood, TX, USA | TL-4200 |
| RT-qPCR primers + probe for SARS-CoV-2 Nucleocapsid RNA | Integrated DNA Technologies, Coralville, IA, USA | none |
| RT -qPCR kit | Meridian Bioscience, Memphis, TN, USA | BIO-78005 |
| Antibodies | Source | Catalog # |
| Mouse monoclonal antibody to hACE2 | Abcam, Cambridge, MA, USA | Ab-89111 |
| Mouse anti-human CD3-FITC | BD Biosciences, SanJose, CA, USA | 555339 |
| Mouse anti-human CD45-FITC | BD Biosciences, SanJose, CA, USA | 347463 |
| Mouse anti-human CD8-PE | BD Biosciences, SanJose, CA, USA | 555635 |
| Mouse anti-human granzyme-B-PE | BD Biosciences, SanJose, CA, USA | 561142 |
| Recombinant human anti-human CD326-PE | Miltenyi Biotec, Berdisch Gladback, Germany | 130-110-999 |
| Mouse anti-human Perforin-PE | Biolegend, San Diego, CA, USA | 353304 |
| Mouse anti-human CD103-FITC | BD Pharmingen, Irvine, CA, USA | 550259 |
| Hamster anti-mouse CD61-PE | Thermo Fisher Scientific, Waltham, MA, USA | 12-0611-82 |
| Mouse anti-human CD31 - Alexa Fluor 594 | Biolegend, San Diego, CA, USA | 303126 |
| Anti-human TMPRSS2 Antibody | Sigma-Aldrich, St. Louis, MO, USA | MABF2158–100UG |
| Goat anti-Mouse IgG Fc-FITC | Southern Biotech, Birmingham, AL, USA | 1013-02 |
| Goat anti mouse IgG-HRP | Santa Cruz Biotechnology, Dallas, TX, USA | Sc-2005 |
| Goat F(ab’)2 anti-mouse lgG2a, Human ads-FITC | Southern Biotech, Birmingham, AL, USA | 1082-02 |
| Goat F(ab’)2 anti-mouse lgG1, Human ads-PE | Southern Biotech, Birmingham, AL, USA | 1072-09 |
| Anti-human IgM antibody-HRP | Bethyl Laboratories, Montgomery, TX, USA | A80-100P |
| Anti-human IgG antibody-HRP | Bethyl Laboratories, Montgomery, TX, USA | A80-104P |
| Anti-human kappa antibody-HRP | Bethyl Laboratories, Montgomery, TX, USA | A80-115P |
| Anti-human lambda antibody-HRP | Bethyl Laboratories, Montgomery, TX, USA | A80-116P |
| ELISA Kits and supplies | Source | Catalog # |
| Human ACE2 ELISA kit PicoKine | Boster Biological Technology, Pleasanton, CA, USA | EK0997 |
| SARS-CoV-2 IgM ELISA Kit (antigen is rS1(RBD) | Bethyl Laboratories, Montgomery, TX, USA | E88-302 |
| SARS-CoV-2 IgG ELISA Kit (antigen is rS1(RBD) | Bethyl Laboratories, Montgomery, TX, USA | E88-301 |
| Coming Costar high-binding ELISA plates | Thermo Fisher Scientific, Waltham, MA, USA | 07-200-721 |
| Biological samples | ||
| Umbilical cord units (HLA-A2.1.HLA-DR0401) | New York Blood Center, Long Island City, NY, USA | n/a |
| DRAGA mice and SARS-CoV-2 virus | ||
| DRAGA mice | WRAIR/NMRC, Silver Spring, MD, USA | n/a |
| SARS-CoV-2 strain USA-WA1/2020 | via U.S. Centers for Disease Control and Prevention, BEI Resources NR-52281 | batch #70033175 |
| Services | ||
| Sequencing PCR amplicons | Eurofins, Inc., Coralville, IA, USA | |
| Tissue sections from paraffin-embedded organs | Histoserv Inc., Germantown, MD, USA | |
| H&E Staining | Histoserv Inc., Germantown, MD, USA | |
| Masson’s Trichrome staining | Histoserv Inc., Germantown, MD, USA | |