KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-CD68 | eBioscience | #14-0688-82 |
| Rabbit polyclonal anti-CD80 | BOSTER | #A00196-1 |
| CD80-PE, human | miltenyi Biotec | #130-117-683 |
| CD206-PE, human | miltenyi Biotec | #130-095-220 |
| Rabbit monoclonal anti-CD163 | Abcam | #ab182422 |
| Rabbit polyclonal anti-IL6 | Affinity | #DF6087 |
| Goat polyclonal anti-ACE2 | R&D system | #AF933 |
| PE-conjugated CD43 | eBioscience | #eBio84-3C1 |
| APC-conjugated CD34 | BD Biosciences | clone 581 |
| PE-conjugated CD68 | Biolegend | clone Y1/82A |
| APC-conjugated CD11b | Biolegend | clone ICRF44 |
| FITC-conjugated CD14 | Biolegend | clone HCD14 |
| Anti-NKX2.1 Antibody | Seven Hills Bioreagents | #WRAB-1231 |
| Anti-FOXA2 Antibody | Santa Cruz | #sc-6554 |
| Anti-SOX2 Antibody | Santa Cruz | #sc-17320 |
| Anti-SP-B Antibody | Seven Hills Bioreagents | #WRAB-48604 |
| Anti-Pro-SP- C Antibody | Seven Hills Bioreagents | #WRAB-9337 |
| Anti-FOXJ1 Antibody | Sigma-Aldrich | #HPA005714-1 |
| Firefly luciferase Monoclonal Antibody (CS 17) | Thermo Fisher Scientific | #35-6700 |
| Recombinant Anti-Firefly Luciferase antibody | Abcam | #ab185924 |
| Mouse Anti-SARS-CoV-Spike antibody | Provided by Dr. Tom Moran | 2B3E5 |
| Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | #A-21202 |
| Alexa Fluor 488 AffiniPure Donkey Anti-Guinea Pig IgG (H+L) | Jackson Immunoresearch Labs | #706-545-148 |
| Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 | Thermo Fisher Scientific | #A-21203 |
| Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 594 conjugate | Thermo Fisher Scientific | #A-21207 |
| Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 647 conjugate | Thermo Fisher Scientific | #A-31573 |
| Donkey anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 647 | Thermo Fisher Scientific | #A-31571 |
| Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 | Thermo Fisher Scientific | #A-21447 |
| Donkey antiChicken IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Jackson Immunoresearch Labs | #703-545-155 |
| Donkey anti-Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 | Thermo Fisher Scientific | #A-21448 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Activin A | R&D Systems | #338-AC-500/CF |
| Y-27632 | MedchemExpress | #HY-10583 |
| Recombinant Human BMP-4 Protein | R & D Systems | #314-BP |
| Recombinant Human bFGF | R&D Systems | #233-FB-500 |
| Dorsomorphin dihydrochloride | R&D Systems | #3093/50 |
| SB431542 | R&D Systems | #1614/50 |
| IWP2 | R&D Systems | #3533/50 |
| CHIR99021 | Cayman Chemical | #13122 |
| Recombinant Human FGF-10 Protein | R&D Systems | #345-FG-250 |
| Recombinant Human KGF/FGF-7 Protein | R&D Systems | #251-KG-01M |
| Retinoic acid | Sigma-Aldrich | #R2625 |
| Dexamethasone | Sigma-Aldrich | #D4902 |
| 8-Bromo-cAMP | Sigma-Aldrich | #B5386 |
| IBMX | Sigma-Aldrich | #15879 |
| Recombinant Human VEGF Protein | R&D Systems | #293-VE-500/CF |
| Recombinant Human IL-3 Protein | R&D Systems | #203-IL-050/CF |
| Recombinant Human M-CSF Protein | R&D Systems | #216-MC-025 |
| Recombinant Human IL4 Protein | R&D Systems | #204-IL-050 |
| IFNγ | R&D Systems | #285-IF-100 |
| LPS | Sigma-Aldrich | #L4391-1MG |
| DAPI | Santa Cruz | #sc-3598 |
| Hoechst 33342 | Sigma-Aldrich | # B2261-100mg |
| Wright-Giemsa Stain | Sigma-Aldrich | #WG16-500ML |
| Culture Medium | ||
| F12 | Gibco Thermo Fisher | #31765035 |
| β-mercaptoethanol | Sigma Aldrich | #M3148 |
| Penicillin- Streptomycin (5,000 U/mL) | Gibco Thermo Fisher | #15070063 |
| MEM Non-Essential Amino Acids Solution (100X) | Gibco Thermo Fisher | #11140050 |
| IMDM | Gibco Thermo Fisher | #21056023 |
| GlutaMAX Supplement | Thermo Fisher Scientific | #35050079 |
| Accutase | Stemcell Technologies | #07920 |
| Matrigel | Corning | #354234 |
| Fibronectin (FN) | Thermo Fisher Scientific | #356008 |
| N2 supplement | Thermo Fisher Scientific | #17502-048 |
| B27 | Thermo Fisher Scientific | #12587-010 |
| DMEM/F12 | Thermo Fisher Scientific | #10565-018 |
| Knockout serum replacement(KOSR) | Thermo Fisher Scientific | #10828-028 |
| FBS | Gibco Thermo Fisher | #10099141C |
| Monothioglycerol | Sigma Aldrich | #M6145 |
| Ascorbic acid | Sigma Aldrich | #A4403 |
| Bovine serum albumin(BSA) | Sigma Aldrich | #A9418 |
| Experimental Models: Cell Lines | Seven Hills Bioreagents | #WRAB-1231 |
| hESC line H1 | Harvard University | #0014 |
| hESC line-RUES2 | The Rockefeller University | #0013 |
| HEK293T | ATCC | #CRL-11268 |
| Vero E6 | ATCC | #CRL-1586 |
| THP-1 | ATCC | #TIB-202 |
| U937 | ATCC | #CRL-1593.2 |
| Mouse embryonic fibroblasts | Global Stem | #GSC-6001G |
| Software and Algorithms | ||
| Cell Ranger | 10X Genomics | https://support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome |
| Scran | Lun ATL, McCarthy DJ, Marioni JC (2016). “A step-by-step workflow for low-level analysis of single-cell RNA- seq data with Bioconductor.” F1000Res, 5, 2122. doi: 10.12688/f1000research.9501.2. | https://bioconductor.org/packages/release/bioc/html/scran.html |
| Rstudio | Rstudio | https://rstudio.com |
| Seurat R package v3.1.4 | 1 | https://satijalab.org/seurat/ |
| DAVID6.8 | LHRI | https://david.ncifcrf.gov/home.jsp |
| Adobe illustrator CC2017 | Adobe | https://www.adobe.com/product/photoshop.html |
| Graphpad Prism 8.0 | Graphpad software | https://www.graphpad.com |
| LEGENDplex v8.0 | Biolegend | https://www.biolegend.com/en-us/legendplex |
| FlowJo v x.0.7 | BD Biosciences | https://www.flowjo.com/ |
| ToppCell Atlas | Toppgene | https://toppgene.cchmc.org/ |