Figure 1.

DOX treatment upregulates LRPPRC at the transcriptional level. (A) Cell Counting Kit-8 assay was performed to detect DOX cytotoxicity in H9C2 cells. (B) Following DOX treatment at IC₃₀ or IC₅₀ for 0, 24, 48, 72 and 96 h, the mRNA levels of LRPPRC, Bcl-2 and Bax were detected via reverse transcription-quantitative PCR. (C) Protein levels of LRPPRC, Bcl-2 and Bax were detected via western blot (left panel), and quantitatively analyzed via ImageJ software (right panel). (D) Via immunofluorescence staining, the expression and localization of LRPPRC (red) and nucleus (blue) were visualized. ^*P<0.05 and ^**P<0.01 vs. 0 h DOX exposure group. LRPPRC, leucine-rich pentatricopeptide repeat-containing; DOX, doxorubicin.