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. 2020 Jul 27;20(4):3301–3309. doi: 10.3892/etm.2020.9054

Figure 2.

Figure 2

Effects of increased CD47 expression on phagocytosis of macrophages and DCs. (A) FACS analysis confirmed the CD47 expression manipulation in Panc02 cells (B) Representative dot plots indicating how the phagocytic index was measured by FACS method (Q3 indicates the Panc02 cells that were phagocytosed by macrophages). (C) Panc02 cells with various expression levels of CD47 were co-cultured with macrophages, and the phagocytic index was measured. Quantified data revealed that macrophages had a higher phagocytic index in CD47-KD Panc02 cells. (D) Anti-CD47 treatment significantly rescued the phagocytic function of macrophages. (E) CD47-overexpressing tumor cells inhibited the phagocytic function of DCs. (F) Anti-CD47 treatment significantly rescued the phagocytic function of DCs. All the experiments were repeated three times.**P<0.01, ***P<0.001 ****P<0.0001. CD47, cluster of differentiation 47; OE, overexpression; WT, wide type; Ctrl, control; KD, knockdown; FACS, fluorescence-activated cell sorting.