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. 2020 Jul 27;20(4):3244–3252. doi: 10.3892/etm.2020.9052

Figure 1.

Figure 1

ROS and apoptosis levels in human degenerated intervertebral disc tissues. Tissues were lysed and ROS measured with MDA methods. Total protein and mRNA were extracted from the tissues in mild (G2-G3) and severe (G4-G5) degeneration. (A) MDA level of different degenerated degrees of disc tissues. (B and C) The protein levels of collagen II, ATF4, CHOP and caspase-9 were determined by (B) Western blot and (C) quantification analysis. (D) mRNA levels of collagen II, ATF4, CHOP and caspase-9 were determined by RT-PCR. The values are mean ± SD of three independent experiments (n=3). (*P<0.05, **P<0.01, ***P<0.001, compared with G2-G3 group). ROS, reactive oxygen species; ATF4, activating transcription factor 4; CHOP, C/EBP homologous protein; RT-PCR, reverse transcription-polymerase chain reaction.