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Number of microglia or progenitors measured by flow cytometry in yolk sac (A), brain rudiment (B), neonates (C) and adult (D). Bar plots represent means ± sem. *p < 0.05, ****p < 0.0001, 2 sample t-test. For microglia progenitors, N = 3–4 biologically independent measures from 6–12 pooled yolk sacs or brain rudiment. For neonate and adult microglia, N = 3–4 animals per group.
