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. 2020 Aug 7;16(8):e1008691. doi: 10.1371/journal.pgen.1008691

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© 2020 Bustamante-Marin et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (A and B) CFAP57 mRNA in normal hTEC cells. Total RNA was extracted at the indicated day of ALI culture. (A) Levels of mRNA for CFAP57 and FOXJ1 rise during ALI culture from RNAseq experiments. (B) Transcript levels of CFAP57 increase, during airway cell differentiation, in parallel to the expression of DNAI1, a ciliary specific gene. PPIA was used as housekeeping gene control. (C) Immunoblots showing increased levels of CFAP57 as ciliated airway cells underwent differentiation along with increased levels of FOXJ1. (D) Immunoblot showing enrichment of CFAP57 in isolated ciliary axonemes (cilia) compared to differentiated cells (Diff) extract. CFAP57 was absent in protein extract from undifferentiated cells (Undiff). DNAI1 was used as a loading control for presence of cilia. Predicted sizes are shown. (E and F) The localization of CFAP57 (magenta) to the cilia was confirmed by immunofluorescence in (E) whole ALI culture and in (F) ciliated cells.