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. 2020 Aug 7;16(8):e1008691. doi: 10.1371/journal.pgen.1008691

Fig 4. CFAP57 silencing in cultured airway cells causes decreased ciliary motility.

Fig 4

(A) The fold changes in CFAP57 after transduction with four specific CFAP57 shRNA sequences normalized to the level in the GFP hTEC cells. shRNA4 has only a weak effect on mRNA levels (n = 3 technical replicates). (A’) Cumulative data of 3 separate experiments using 3 different donors of primary hTEC (n = 3 biological replicates). (B) Immunoblot on the shRNA cells that shows loss of CFAP57 protein in three of the hTEC cultures. An immunoblot with actin antibody was used as a loading control. (C) Immunofluorescent staining of hTEC with antibodies to acetylated α-tubulin (green) and CFAP57 (magenta) after shRNA-targeted silencing with shRNA2 of CFAP57. (D) Ciliary beat frequency of CFAP57 silenced cells showing altered beat frequency. Each dot represents random area measurement (n = 3 replicates). Scale bar = 10 μm.