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. 2020 Aug 20;15(8):e0227157. doi: 10.1371/journal.pone.0227157

Fig 1. Pertussis toxin treatment effects vary depending on the day of infection.

Fig 1

OT-I-GFP CD8 T cells were adoptively transferred into naïve hosts one day prior to infection with influenza virus expressing the SIINFEKL peptide. CD8 T cell motility was tracked by IVMPM and the data analyzed using Volocity software. Ptx was administered intravenously 5h prior to imaging. The motility parameters for Velocity (A), Displacement (B), and Confinement (C) are plotted. The mean and standard deviations appear in red. A non-parametric Mann-Whitney test with Bonferroni correction was applied to each pair of data sets for each day of infection. *** p < 0.01; **** p < 0.0001. N = 3–5 animals per group, 33 total. Surgical set up takes about 15 minutes, and the animal is on the scope for about an hour—1 1/2 hrs resulting in 30–45 mins of usable data.