Fig 4. Administration of exogenous OVA SIINFEKL peptide restores T cell arrest on day 9.

Host animals received adoptively transferred CD8 T cells and were infected with influenza as described in Material and Methods. 100μg OVA SIINFEKLpeptides in saline were injected intravenously during the imaging. CD8 T cell motility was tracked by IVMPM and the data analyzed using Volocity software. The motility parameters for velocity (A), Displacement (B), and Confinement Index (C) are plotted. The mean and standard deviations appear in red. A non-parametric Mann-Whitney test corrected for multiple comparisons was applied to each pair of data sets for each day of infection. ns = not significant; **** p < 0.0001. N = 5 animals per group, 20 total. Surgical set up takes about 15 minutes, and the animal is on the scope for about an hour—1 1/2 hrs resulting in 30–45 mins of usable data.