Table 1. Primers used in qRT-PCR (F: forward; R: reverse).

Gene	Primer sequence (5′–3′)	Tm (°C)	Product size (bp)
Alp	F: CCAACTCTTTTGTGCCAGAGA	58	110
	R: GGCTACATTGGTGTTGAGCTTTT	60
Runx2	F: CTTTACCTACACCCCGCCAG	60	116
	R: GTCCACTCTGGCTTTGGGAA	60
Ptch1	F: CCAGCGGCTACCTACTGATG	60	150
	R: TGCCAATCAAGGAGCAGAGG	60
Sox9	F: TGAAGAACGGACAAGCGGAG	60	198
	R: CAGCTTGCACGTCGGTTTTG	60
Mmp13	F: GGAGCCCTGATGTTTCCCAT	60	165
	R: ATCAAGGGATAGGGCTGGGT	60
Gapdh	F: CATGGCCTTCCGTGTTCCTA	60	172
	R: GTTGAAGTCGCAGGAGACAAC	60