Table 1. Yield and purity of SCF-Lα-GCSF and GCSF-Lα-SCF after four processing steps.
| Protein purification step | Characteristic (%) | SCF-Lα-GCSF | GCSF-Lα-SCF |
|---|---|---|---|
| Refolding | Yield1 | 93.2 ± 2.9 | 91.7 ± 2.1 |
| Purity2 | 24.2 ± 1.0 | 35.8 ± 2.2 | |
| I Chromatography step (anion exchange) | Yield1 | 7.3 ± 0.5 | 6.1 ± 0.3 |
| Purity2 | 63.5 ± 2.5 | 58.6 ± 1.4 | |
| II Chromatography step (mixed-mode) | Yield1 | 1.9 ± 0.4 | 1.8 ± 0.3 |
| Purity2 | 77.5 ± 3.0 | 67.2 ± 2.2 | |
| III Chromatography step (cation exchange) | Yield1 | 1.4 ± 0.1 | 1.2 ± 0.2 |
| Purity2 | 92.2 ± 2.1 | 90.4 ± 1.6 |
Notes:
1
Protein concentrations were determined by the Bradford method (Bradford, 1976) using bovine serum albumin as a standard.
2
Purity of the protein was determined by RP-HPLC.
Each value represents the mean of three independent analyses ± standard deviation (SD).