Table 2. Summary of characteristics of the fusion proteins and respective monomers.

Characteristic	SCF-Lα-GCSF	GCSF-Lα-SCF	G-CSF monomer	SCF monomer
Theoretical MW (kDa)	4,2369.74	4,2369.74	18,798.85	18,656.49
Experimental MW (kDa)	4,2370.48	4,2370.70	ND	ND
SEC size (kDa)	>150	>150	<13.7	>44.3
Biological activity on G-NFS-60 cell linea (IU/mmol)	0.63 × 1012	0.25 × 1012	1.88 × 1012	ND
Biological activity on M-07e cell lineb (IU/mmol)	6.70 × 109	12.80 × 109	ND	9.33 × 109
Association and dissociation constants of the protein and the SCF receptor (TIRE data)
ka (M−1 s−1)	7.77 × 104	1.14 × 106	ND	1.37 × 104
kd (s−1)	1.39 ×10−2	6.46 × 10−1	ND	1.43 × 10−2
Ka (M−1)	5.59 × 106	1.76 × 106	ND	9.50 × 105
(Kd) (M)	1.78 × 10−7	5.67 × 10−7	ND	1.04 × 10−6
Association and dissociation constants of the protein and the G-CSF receptor (TIRE data)
ka (M−1 s−1)	8.50 × 104c	No interaction	7.50 × 105c	ND
kd (s−1)	1.25 × 10−3c		1.05 × 10−2c	ND
Ka (M−1)	6.80 × 107c		7.14 × 107c	ND
Kd (M)	0.15 × 10−7c		0.14 × 10−7c	ND

Notes:

^aCell line expressing the G-CSF receptor.

^bCell line expressing the SCF receptor.

^cThe data previously described in Balevicius et al. (2019).

ND, not determined.