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. 2020 Aug 10;16(8):e1008754. doi: 10.1371/journal.ppat.1008754

Fig 3. Kayak and Puckered expressions are induced by DENV2, ZIKV and CHIKV infection, and Puckered depletion restricts ZIKV infection in salivary glands.

Fig 3

(A) Kayak (Kay) and (B) Puckered (Puc) expressions in salivary glands at 3, 7 and 14 days post oral infection with DENV2, ZIKV and CHIKV. Gene expression was quantified in pools of 10 salivary glands. Actin expression was used for normalization. Bars show arithmetic means ± s.e.m. from three biological replicates. (C) Effect of Puc depletion on infection in salivary glands at 7 days post ZIKV inoculation. Four days prior infection, mosquitoes were injected with dsRNA. Bars show geometric means ± 95% C.I. from 20 individual pair of salivary glands. Each dot represents one sample. dsCtrl, dsRNA against LacZ, dsPuc, dsRNA against Puc. *, p < 0.05, as determined by Dunnett’s test within time points with mock infection as control (A, B) or unpaired t-test (C).