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. 2020 Aug 10;16(8):e1008754. doi: 10.1371/journal.ppat.1008754

Fig 5. Functions of TEP20 and Dronc in SGs.

Fig 5

DsRNA-injected mosquitoes were inoculated with ZIKV and analyzed 10 days later. Controls were injected with dsCtrl. (A) Impact of TEP20 depletion on expression of the AMPs: CecA, DefA and DefD. Gene expression was quantified in pools of 10 salivary glands. Actin expression was used for normalization. Bars show arithmetic means ± s.e.m. from three biological replicates. (B) Apoptotic staining in SGs upon depletion of Dronc (dsDronc), TEP20 (dsTEP20) and both simultaneously (dsDronc/dsTEP20). Apoptotic cells were detected via TUNEL staining (red) and nuclei were stained using DAPI (blue). Scale bar, 200 μm. (C) Number of apoptotic cells in salivary glands upon depletion of Dronc, TEP20 and both. Cells were counted on twelve 0.05 mm2 areas observed from two SGs per condition. dsCtrl, dsRNA against LacZ; dsTEP20, dsRNA against TEP20; dsDronc, dsRNA against Dronc; CecA, Cecropin A; DefA; Defensin A, DefD, Defensin D. ***, p < 0.001, as determined by Tukey’s post-hoc test.