Fig 4. Gene expression of keratins.

The UROtsa parent (Ai-viii), As#3 (Bi-viii), and As#4 (Ci-viii) were treated with either DMSO (control), troglitizone (TG, 10 μM), PD153035 (PD, 1 μM), or TG and PD (TG+PD) for 72 hr. Real time RT-PCR analysis was performed to verify gene expression (A, B, Ci-viii). Gene expression was normalized to β-actin and are plotted as fold-change relative to the DMSO control. Triplicate measurements of gene levels were performed and are reported as mean ± SEM. Ordinary one-way ANOVA was performed followed by Tukey’s post-hoc test. Bars with differing letters indicate significant differences (p < 0.05).