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. 2020 Aug 21;15(8):e0237932. doi: 10.1371/journal.pone.0237932

Fig 6. Recombinant FUD prevented TGFβ2 induced increase in fibronectin and EDA+ fibronectin fibrillogenesis.

Fig 6

Confluent HTM cultures (N25TM-8) were untreated (A-C) or treated with 2μM FUD (D-F), 2ng/ml TGFβ2 (G-I) or both FUD and TGFβ2 (J-L) for 4 days. Cells were then fixed and double labeled with a polyclonal fibronectin sera (A, D, G and J) or an antibody (Ist-9) that recognizes the EDA domain of fibronectin (B, E, H and K). Arrow heads in merged fibronectin and EDA+ fibronectin images (C, F, I and L) indicated where fibronectin and EDA+ fibronectin labeling coincide. Similar results were seen with one other cell strain. Bar = 50μm. (I) Schematic of fibronectin indicates that FUD binds the N-terminal end of fibronectin and location of the epitopes recognized by the monoclonal antibodies Ist-9 and BC-1.