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. 2020 Aug 11;16(8):e1008707. doi: 10.1371/journal.ppat.1008707

Fig 11. Zinc-coordinating histidines are required for biofilm formation.

Fig 11

Biofilm formation by an MR/P locked-ON mutant, a locked-ON mrpH double mutant, and the double mutant complemented with wild-type or mutated mrpH expressed from plasmid pGEN-Pmrp-mrpH (+mrpH). pGEN-Pmrp-luxCDABE (EV) is a negative control plasmid and has luciferase genes under the control of the native mrp promoter. Significance compared against pGEN-Pmrp-mrpH using one-way ANOVA with Dunnett’s multiple comparisons test. Compared with the negative (EV) control, these mutants were not significantly different: H72A, H74A, H117A, and R118A; E127A P = 0.0130.