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. 2020 Aug 19;15(8):e0237181. doi: 10.1371/journal.pone.0237181

Table 1. Subtractive proteomics analysis scheme.

Sl. No. Steps No. of proteins Protein sets
1 Total number of proteins 4822 Set 0 (S1 File)
2 Non-paralogous (>60% identical) in CD-Hit 4729 Set 1 (S2 File)
3 proteins with >100 amino acids 4123 Set 2 (S3 File)
4 Number of proteins nonhomologous to H. sapiens using BLASTp (E value 10−3) 3164 Set 3 (S4 File)
5 Essential proteins in DEG 15.2 server (E value ≤10−100, Bit score >100) 1107 Set 4 (S5 File)
6 Essential Proteins involved only in unique metabolic pathways (KAAS at KEGG) 96 Set 5 (S9 File)
7 Essential proteins found to be novel in DrugBank 5.1.0 (using default parameters) 41 Set 6 (S10 File)
8 Novel drug target proteins non-homologous to ‘anti-targets’ using BLASTp (E value <0.005, Identity <25%, Query length >30%) 41 Set 7 (S11 File)
9 Essential cytoplasmic proteins using PSORTb, CELLO, ngLOC, PSLpred 16 Set 8 (S12 File)
10 Proteins showing <45% similarity with human microflora proteins 9 Set 9 (S13 File)
11 Identified virulence associated novel proteins by VFDB analysis 2 Set 10 (Table 4)
12 Essential membrane proteins using PSORTb, CELLO, ngLOC, PSLpred 25 Set 11 (S14 File)
13 Identified vaccine targets having less similarities with human microflora proteins and antigenicity 2 Set 12 (Table 7)