Skip to main content
. 2020 Aug 20;15(8):e0235787. doi: 10.1371/journal.pone.0235787

Fig 4.

Fig 4

Samples were analyzed by (a-e) C18 chromatography (f-j) and HILIC chromatography. The (a) base peak chromatogram of C18 analyzed samples shows an abundance of polar compounds eluting in the void volume, including (b-e) significantly expressed components. To reliably determine these polar compounds, HILIC chromatography was used (f-j). The putative Leu, Ile amino acids identified in (e) C18, were determined to be (j) β-alanine-betaine.