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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Methods Cell Biol. 2019 Dec 10;156:15–43. doi: 10.1016/bs.mcb.2019.10.009

Figure 1: Osteogenic monolayer culture of MSCs for generation of OECM:

Figure 1:

Panel a: hMSCs at the appropriate confluency for reseeding into osteogenic cultures. Panel b: hMSCs at the appropriate confluency for initiation of osteogenic assays. Panel c: Crystal violet-stained monolayers of osteogenic hMSCs detaching form the tissue culture plastic. Panel d: Use of rotary burr to generate enhanced attachment sites (inset) at the edges of wells prior to osteogenic culture. Panel e: hMSCs stained with ARS cultured in osteogenic differentiation media (left) or control CCM media (right). For panel a,b,e: bar = 200 mm. For panel c: bar = 10 mm. For panel d: bar = 5 mm.