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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Methods Cell Biol. 2019 Dec 10;156:15–43. doi: 10.1016/bs.mcb.2019.10.009

Table 1:

A selection of plastic adherent osteogenic cell lines from various sources.

Cell type and species Osteogenic conditions Ref.
Human bone marrow derived MSCs α-MEM, FBS, β-glycerophosphate, ascorbate, dexamethasone (C. A. Gregory, Gunn, Peister, & Prockop, 2004; Wei et al., 2017)
Human bone marrow derived MSCs α-MEM, FBS, β-glycerophosphate, ascorbate, GW9662 (Krause et al., 2010; Zeitouni et al., 2012)
Chicken bone marrow derived MSCs DMEM, FBS, β-glycerophosphate, ascorbate, dexamethasone (Bai et al., 2013)
Rabbit bone marrow derived MSCs DMEM-LG, FBS, β-glycerophosphate, ascorbate, dexamethasone (Solchaga, Johnstone, Yoo, Goldberg, & Caplan, 1999)
Canine various tissues MSCs α-MEM, FBS, β-glycerophosphate, ascorbate, dexamethasone, BMP2 (Bearden et al., 2017)
Rat bone marrow derived MSCs α-MEM, FBS, β-glycerophosphate, ascorbate, dexamethasone (Javazon, Colter, Schwarz, & Prockop, 2001)
Mouse bone marrow derived MSCs IMDM, FBS/HS, β-glycerophosphate, ascorbate, dexamethasone, thyroxine (Peister et al., 2004)
Human adipose derived MSCs DMEM, FBS, β-glycerophosphate, ascorbate, dexamethasone or DHVD3 (Zuk et al., 2002)
Murine adipose derived MSCs α-MEM, FBS, β-glycerophosphate, ascorbate, dexamethasone (Xie et al., 2018)
Human iPSC-derived MSCs α-MEM, FBS, β-glycerophosphate, ascorbate, dexamethasone (Zhao et al., 2015)
Human MG63 osteosarcoma (OS) cells α-MEM, FBS, β-glycerophosphate, ascorbate, dexamethasone (C. A. Gregory et al., 2004)
Human SAOS OS cells McCoy’s 5A, β-glycerophosphate, ascorbate (Jiang et al., 2013)
Human MNNG/HOS OS cells StemPro (ThermoFisher, Waltham, MA) Osteogenesis Kit (Martins-Neves et al., 2012)
Murine MC3T3 OP line DMEM-LG, FBS, β-glycerophosphate, ascorbate, dexamethasone (Liu et al., 2014)
Murine MOSJ osteosarcoma cells α-MEM, FBS, β-glycerophosphate, ascorbate, dexamethasone (Krause, Ryan, Clough, & Gregory, 2014)

α-MEM: alpha minimal essential medium, DMEM: Dulbecco’s minimal essential medium, LG: low glucose, FBS: fetal bovine serum, BMP2: bone morphogenic protein 2, IMDM: Iscove’s modified Dulbecco media. DHVD3: dihydroxy vitamin D3, iPSC: induced pluripotent cells