Table 2:
A selection of commonly employed osteogenic biomarkers for qRTPCR
| Transcript | Stagea | SYBR primersb | Ref. | TaqMan assay |
|---|---|---|---|---|
| GAPDH | Normalization | GAGCCACATCGCTCAGACA CCTCTTCAAGGGGTCTAC |
(Carraro, Albertin, Forneris, & Nussdorfer, 2005) | Hs01548420_m1 |
| Alkaline phosphatase | Day 8-14 | GACCCTTGACCCCCACAAT GCTCGTACTGCATGTCCCCT |
(Zhao et al., 2015) | Hs01029144_m1 |
| BMP2 | Day 2-5 | CCCAGCGTGAAAAGAGAG GAGACCGCAGTCCGTCTA |
(McNeill et al., 2018) | Hs00272516_m1 |
| Collagen I | Day 2-21 | GAACGCGTGTCACCTTGT GTCAACGCGTGTCATCCCTTGT |
(Zhao et al., 2015) | Hs00164004_m1 |
| Collagen X | Day 8-21 | AATGCCTGTGTCTGCTTTTAC ACAAGTAAAGATTCCAGTCCT |
(Alaseem et al., 2015) | Hs00166657_m1 |
| Osteocalcin | Day 14-21 | TGAGAGCCCTCACACTCC CGCCTGGGTCTCTTCACTAC |
(Salem et al., 2014) | Hs01587814_g1 |
| Osterix | Day 2-5 | GTGGGCAGCTAGAAGGGAGT AATTAGGGCAGTCGCAGGA |
(Schaap-Oziemlak et al., 2010) | Hs00541729_m1 |
| Runx2 | Day 2-5 | GCAAGGTTCAACGATCTGAGA TCCCCGAGGTCCATCTACTG |
(Schaap-Oziemlak et al., 2010) | Hs01047973_m1 |
a
approximate time-window when maximally activated, but dependent on cells assayed.
b
primers provided for human cells.