Skip to main content
. Author manuscript; available in PMC: 2021 Aug 11.
Published in final edited form as: Biochemistry. 2020 Aug 2;59(31):2842–2848. doi: 10.1021/acs.biochem.0c00441

Figure 5.

Figure 5.

Effects of non-specific linear DNA competitors on the yields of NER dual incision products with linearized plasmid substrates containing single (+)-cis-B[a]P-dG lesions. (A) Digestion of the circular 32P-labeled plasmid by ScaI to form the linearized plasmid substrate (1); digestion of the linearized substrate (1) with BsrBI to form a mixture of linear fragments (2). (B) Digestion of the non-radioactive pUC19 plasmid by ScaI to form the linearized plasmid (3); digestion of the linearized plasmid (3) by BsrBI to form a mixture of linear DNA fragments (4). (C) NER dual incision product yield after incubation of the linear DNA substrate (1) or (2), in the presence of non-specific competitor DNA fragments (3), or (4), derived from the digestion of 0.2 nM DNA substrates (1) or (3) as shown in panels A and B. Incubation time: 30 min in each case.