Table 1.
Expression of putative tumor stem cell markers in the NCI60 tumor cell line panel. Expression levels of CD15/CD24/CD44 or expression levels of CD133 and ALDH activity were analyzed by FACS analysis (50,000 cells/sample), and the size of marker positive and marker negative populations was determined using FlowJo (Treestar Inc). For each marker, the size of the marker positive subpopulation was determined (percent positive cells), and data were centered to the mean. FACS analysis was performed in triplicate
| Tumor Type | Cell Line Name | CD44+ | CD24+ | CD133+ | CD15+ | ALDH+ | CD44+ CD24[minus] | CFU (2-D) | soft agar assay |
|---|---|---|---|---|---|---|---|---|---|
| hematopoeitic | CCRF-CEM | 8.44 | 0.53 | 0.04 | 7.59 | 0.03 | 8.29 | 6 | |
| hematopoeitic | HL-60 | 43.01 | 0.03 | 0.07 | 56.77 | 0.04 | 42.98 | 32 | |
| hematopoeitic | K-562 | 0.13 | 0.31 | 0.09 | 44.91 | 12.86 | 0.11 | 64 | |
| hematopoeitic | MOLT-4 | 0.57 | 0.49 | 0.05 | 0.39 | 1.32 | 0.54 | 28 | |
| hematopoeitic | RPMI-8226 | 0.09 | 0.19 | 0.05 | 1.68 | 0.00 | 0.06 | 18 | |
| hematopoeitic | SR | 0.08 | 0.22 | 0.07 | 0.21 | 0.00 | 0.06 | 12 | |
| NSC Lung | A549/ATCC | 84.41 | 71.73 | 3.64 | 2.05 | 2.55 | 17.84 | 94 | 63 |
| NSC Lung | EKVX | 77.00 | 81.47 | 1.95 | 0.48 | 9.12 | 7.86 | 35 | 0 |
| NSC Lung | HOP-62 | 81.41 | 4.35 | 1.91 | 0.87 | 2.46 | 77.46 | 45 | 37 |
| NSC Lung | HOP-92 | 95.44 | 1.61 | 0.25 | 1.85 | 0.48 | 93.99 | 15 | |
| NSC Lung | NCI-H226 | 89.32 | 16.41 | 0.15 | 0.90 | 14.76 | 74.05 | 20 | 0 |
| NSC Lung | NCI-H23 | 30.95 | 4.30 | 0.11 | 43.51 | 0.01 | 27.97 | 42 | 27 |
| NSC Lung | NCI-H322M | 54.49 | 52.65 | 3.45 | 88.27 | 0.01 | 23.09 | 20 | 29 |
| NSC Lung | NCI-H460 | 67.44 | 27.81 | 0.38 | 50.55 | 1.61 | 49.94 | 161 | 55 |
| NSC Lung | NCI-H522 | 1.40 | 81.25 | 0.18 | 2.41 | 35.74 | 0.02 | 29 | 1 |
| Colon | COLO 205 | 60.97 | 58.36 | 5.78 | 75.50 | 0.04 | 18.39 | 43 | 77 |
| Colon | HCC-2998 | 3.19 | 5.35 | 0.60 | 54.26 | 0.04 | 1.95 | 8 | 56 |
| Colon | HCT-116 | 58.62 | 0.78 | 63.18 | 0.14 | 0.56 | 57.93 | 126 | 102 |
| Colon | HCT-15 | 14.14 | 22.41 | 0.17 | 49.74 | 0.03 | 7.44 | 120 | 63 |
| Colon | HT-29 | 71.39 | 55.45 | 74.51 | 52.78 | 38.80 | 20.99 | 112 | 71 |
| Colon | KM12 | 48.87 | 9.06 | 10.71 | 10.65 | 3.42 | 40.29 | 29 | 37 |
| Colon | SW-620 | 11.49 | 52.84 | 22.04 | 90.05 | 0.11 | 2.25 | 158 | 60 |
| CNS | SF-268 | 86.02 | 18.77 | 1.39 | 0.25 | 0.03 | 68.12 | 37 | 8 |
| CNS | SF-295 | 98.07 | 24.70 | 0.09 | 4.37 | 0.03 | 73.56 | 65 | 54 |
| CNS | SF-539 | 84.22 | 33.08 | 0.13 | 0.48 | 0.00 | 52.76 | 30 | 18 |
| CNS | SNB-19 | 65.79 | 20.00 | 0.50 | 1.06 | 0.01 | 47.64 | 90 | 75 |
| CNS | SNB-75 | 88.23 | 20.36 | 0.25 | 0.39 | 0.69 | 68.13 | 10 | 8 |
| CNS | U251 | 77.53 | 4.98 | 0.55 | 0.12 | 0.75 | 72.82 | 38 | 99 |
| Melanoma | LOX IMVI | 67.54 | 2.11 | 0.48 | 22.45 | 2.59 | 65.84 | 2 | 50 |
| Melanoma | M14 | 95.03 | 2.80 | 0.45 | 0.11 | 2.27 | 92.27 | 74 | 92 |
| Melanoma | MALME-3M | 97.37 | 0.19 | 0.30 | 0.19 | 6.56 | 97.20 | 29 | 13 |
| Melanoma | SK-MEL-2 | 71.24 | 0.60 | 1.92 | 0.19 | 1.36 | 70.79 | 14 | 8 |
| Melanoma | SK-MEL-28 | 90.39 | 1.29 | 21.25 | 0.30 | 9.12 | 89.18 | 18 | 36 |
| Melanoma | SK-MEL-5 | 83.09 | 3.71 | 1.74 | 0.50 | 0.00 | 79.89 | 29 | 37 |
| Melanoma | UACC-257 | 87.94 | 12.52 | 3.84 | 0.26 | 8.62 | 75.66 | 27 | 22 |
| Melanoma | UACC-62 | 67.28 | 4.24 | 0.21 | 0.58 | 0.15 | 63.35 | 34 | 19 |
| Melanoma | MDA-MB-435 | 76.91 | 0.77 | 0.40 | 0.95 | 6.62 | 76.24 | 117 | |
| Ovarian | IGROV-1 | 66.38 | 78.70 | 6.81 | 19.77 | 9.86 | 2.29 | 10 | 1 |
| Ovarian | OVCAR-3 | 12.70 | 91.33 | 22.46 | 88.38 | 0.97 | 0.05 | 19 | 0 |
| Ovarian | OVCAR-4 | 77.81 | 11.98 | 1.77 | 0.35 | 0.65 | 44.55 | 22 | 0 |
| Ovarian | OVCAR-5 | 98.97 | 54.69 | 19.36 | 0.54 | 0.04 | 44.42 | 41 | 5 |
| Ovarian | OVCAR-8 | 89.94 | 94.43 | 0.09 | 93.32 | 0.00 | 2.61 | 118 | 84 |
| Ovarian | SK-OV-3 | 90.74 | 58.49 | 0.78 | 8.16 | 0.00 | 34 | 19 | |
| Ovarian | NCI/ADR-RES | 73.62 | 64.05 | 0.54 | 84.19 | 0.96 | 19.67 | 19 | |
| Renal | 786-0 | 94.59 | 96.32 | 0.93 | 15.01 | 2.58 | 0.99 | 37 | 2 |
| Renal | A-498 | 77.86 | 43.71 | 0.39 | 7.03 | 5.08 | 37.07 | 20 | 0 |
| Renal | ACHN | 77.55 | 81.56 | 0.45 | 0.44 | 16.48 | 1.16 | 68 | 1 |
| Renal | CAKI-1 | 78.71 | 67.50 | 0.23 | 3.16 | 13.16 | 20.40 | 39 | 0 |
| Renal | RXF-393 | 93.59 | 63.98 | 0.20 | 5.34 | 0.00 | 30.26 | 3 | 1 |
| Renal | SN12C | 14.63 | 93.43 | 57.47 | 41.57 | 0.00 | 0.04 | 17 | 34 |
| Renal | TK-10 | 94.31 | 91.45 | 0.75 | 91.84 | 1.66 | 6.25 | 53 | 4 |
| Renal | UO-31 | 89.70 | 21.47 | 5.93 | 4.98 | 0.02 | 68.74 | 28 | 0 |
| Prostate | PC-3 | 74.93 | 14.00 | 0.78 | 45.00 | 22.32 | 62.61 | 26 | |
| Prostate | DU145 | 66.56 | 38.92 | 0.87 | 68.37 | 0.08 | 34.27 | 53 | |
| Breast | MCF7 | 1.37 | 20.44 | 1.24 | 33.66 | 0.40 | 0.11 | 74 | |
| Breast | MDA MB 231/ATCC | 90.19 | 4.22 | 0.38 | 27.39 | 0.15 | 86.33 | 100 | |
| Breast | Hs-578T | 67.80 | 28.23 | 1.03 | 0.45 | 0.10 | 41.06 | 14 | |
| Breast | BT-549 | 45.27 | 12.91 | 4.54 | 1.01 | 0.04 | 33.40 | 68 | |
| Breast | T-47D | 24.21 | 72.03 | 1.11 | 69.69 | 0.03 | 2.67 | 74 |