Table 3. Available studies^* of EVs secreted by primary human macrophages derived from circulating monocytes.

Research objective	EV isolation methods	EV characterization methods	Filtration as an extra isolation step	Internalization assay	References
Functional study	UC	TEM, FC, WB and Sucrose gradient assay	No	No	[65]
Functional study	UC	TEM, LP, Sucrose gradient assay and PT	Yes	Yes	[66]
Proteomic study	UC	NTA, TEM, WB and PT	Yes	No	[67]
Proteomic study	UC	TEM, WB and PT	Yes	No	[17]
Micro RNA profile	UC	Iodixanol gradient assay, AChE activity and WB	No	No	[68]
Functional study	EVs isolation kit	NTA, TEM and WB	No	No	[70]
Functional study	EVs isolation kit	TEM and WB	Yes	No	[69]
Functional study	UC	NTA, TEM, FC and WB	Yes	No	[18]
Functional study	UC	NTA, TEM and WB	Yes	No	[71]
Functional study	UC	NTA, TEM and WB	Yes	No	[64]
Functional study	UC	NTA, TEM and FC	No	No	[72]

AChE: acetylcholinesterase activity; LP: Lipidome; FC: Flow citometry; PT: Proteomic

*PubMed, June 2020