KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-H3K27ac | Active Motif | Active Motif Cat# 39134, RRID:AB_2722569 |
| Rabbit anti-CoRest (C. floridanus) | This paper | S.L. Berger - University of Pennsylvania, Cat# Berger_CF_CoRest_001, RRID:AB_2811209 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Minor C. floridanus workers (d0, d5, d10) | Lab-reared from field collected colonies | N/A |
| Major C. floridanus workers (d0, d5, d10, TSA-treated, DMSO-treated, RNAi-injected+TSA/DMSO treated) | Lab-reared from field collected colonies | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Trizol Reagent | Invitrogen | Cat #15596026 |
| in vivo JET-PEI | Polypus | Cat #201–10G |
| Trichostatin A | Sigma-Aldrich | Cat #T1952 |
| Agencourt AMPure XP | Beckman Coulter | Cat #A63880 |
| Juvenile Hormone III-d3 | Toronto Research Chemicals | Cat #E589402 |
| Critical Commercial Assays | ||
| NEBNext® Ultra™ II Directional RNA Library Prep | NEB | Cat #E7706 |
| NEBNext® Ultra™ II DNA Library Prep | NEB | Cat #E7645 |
| SYBR green master Mix | Roche | Cat #04 707 516 001 |
| High capacity cDNA reverse transcription kit | Applied Biosystems | Cat #4368814 |
| TURBO™ DNase (2 U/μL) | Invitrogen | Cat #AM2239 |
| Dynabeads™ Protein A | Invitrogen | Cat #10002D |
| Deposited Data | ||
| Raw sequencing data | SRA | BioProject: PRJNA530332 |
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| Camponotus floridanus | Florida Keys, USA | |
| Oligonucleotides | ||
| CoRest_dsiRNA1 Sense: 5’ rCrCrUrGrArUrGrArArUrGrGrArCrArGrUrUrGrArArGrATA 3’ Antisense: 5’ rUrArUrCrUrUrCrArArCrUrGrUrCrCrArUrUrCrArUrCrArGrGrArA 3’ | IDT | N/A |
| CoRest_dsiRNA1259 Sense: 5’ rCrUrGrGrGrUrCrUrGrGrArCrArArGrCrUrArCrUrArArATA 3’ Antisense: 5’ rUrArUrUrUrArGrUrArGrCrUrUrGrUrCrCrArGrArCrCrCrArGrUrG 3’ | IDT | N/A |
| CFLO_JHe Forward: GTGAATCCAGTAACAACACAAC Reverse: TTCGGCCATTCTACACCAG | GeneWiz | N/A |
| CFLO_JHeh Forward: TCGGTGCTTTTATTCCTCAAC Reverse: TCCAAAATATATGCTGCCAGTC | GeneWiz | N/A |
| CFLO_ttk Forward: TTGTTGGCCATCCCGATAAG Reverse: TAAGCGCAGAACCGAGTAATG | GeneWiz | N/A |
| CFLO_CoRest_cs1 Forward: ATCGCATTCGGCAAATGTTAC Reverse: CGTCAATTTACGTGCTTGTCTATC | IDT | N/A |
| CFLO_CoRest_cs2 Forward: CCTGTTAATGAACGTCGATTGG Reverse: TCCTAATGCCTGTTCTCCATTAT | IDT | N/A |
| Recombinant DNA | ||
| Software and Algorithms | ||
| Trimmomatic | Bolger et al., 2014 | |
| Bowtie2 v2.2.6 | Langmead and Salzberg, 2012 | |
| STAR | Dobin et al., 2013 | |
| Diffbind | Stark and Brown, 2011 | |
| DESeq2 | Love et al., 2014 | |
| MACS2 version 2.1 | Zhang et al., 2008 | |
| MEME | Bailey et al., 2006 | |
| GeneOverlap | Shen and Sinai, 2013 | |
| OrderedList | Yang et al., 2008 | |
| Blast2GO | Conesa et al., 2005 | |
| topGO | Alexa and Rahnenfuhrer, 2010) | |
| Other | ||