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. 2020 Aug 12;26:e922900-1–e922900-14. doi: 10.12659/MSM.922900

Figure 2.

Figure 2

Effect of circ_0008193 overexpression in human lung adenocarcinoma (LUAD) cells under hypoxia in vitro. (A) Real-time quantitative polymerase chain reaction (RT-qPCR)-confirmed circ_0008193 level in hypoxic A549 and H1975 cells transfected with vectors carrying circ_0008193 or not (vector or circ_0008193). (B) 3-(4, 5-Dimethylthiazol-2-yl)-2, 5 diphenyltetrazolium bromide (MTT) assay evaluated cell viability; (C, D) special kits assessed glucose and lactate concentrations; (E, F) western blotting measured hexokinase II (HK2) and lactate dehydrogenase A (LDHA) expression; (G–J) transwell determined numbers of migrating and invading cells; (K, L) western blotting also measured matrix metalloproteinase 2 (MMP2) and MMP9 levels in transfected A549 and H1975 cells after hypoxia treatment for 48 h. *** P<0.001 from three independent experiments.