(a) The nuclei in tenocytes becomes more ovoid to round in shape without conspicuous cytoplasm; 1–2 clusters of capillaries seen per 10 high power fields. Separation of fibers with loss of demarcation of bundles and clear loss of normal polarization pattern. Control 5, H&E, ×200; (b) Separation of individual fibers with maintenance of demarcated bundles. PRP 4, Masson Trichrom, ×200; (c) Stainable mucin between fibers but bundles still discrete. ACS 4, Alcian blue, polarized light, ×200; (d) Type I/III collagen, PRP 1, Picrosirius Red, polarized light, ×100; (e) Type I/III collagen, ACS 2, Picrosirius Red, polarized light, ×100