Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus male/female) |
C57BL/6J | Jackson Laboratories | IMSR Cat# JAX:000664, RRID:IMSR_JAX:000664 | Wild type mouse line |
| Cell line (Mus musculus) |
RAW 264.7 | ATCC | TIB-71 | Cryopreserved monocyte cell line |
| Antibody | Anti-Integrin aVb5 (Mouse monoclonal) | Abcam | ab78289, RRID:AB_1566022 | Neutralizing antibody (1:1000) |
| Peptide, recombinant protein | Irisin | Lake Pharma Kim et al., 2018 | N/A (custom) | 10 his-tag recombinant from HEK 293 cells |
| Peptide, recombinant protein | RANKL, recombinant human | PeproTech | 310–01 | Osteoclast growth factor |
| Peptide, recombinant protein | M-CSF, recombinant murine | PeproTech | 315–02 | Osteoclast growth factor |
| Commercial assay or kit | Acid Phosphatase Kit | Sigma-Aldrich | 387A | TRAP assay |
| Commercial assay or kit | Corning Osteo Assay | VWR | 89184–614 | Resorption assay |
| Commercial assay or kit | OsteoLyse Assay Kit | Lonza | PA-1500 | Resorption assay |
| Software, algorithm | Prism 8 | Graphpad | RRID:SCR_002798 | Statistics/graphing software |
| Software, algorithm | FIJI | NIH | RRID:SCR_002285 | Image analysis software |