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. 2020 Apr 1;123(5):1828–1837. doi: 10.1152/jn.00527.2019

Fig. 3.

Fig. 3.

Excitatory amino acid transporter 5 (EAAT5)-mediated feedback inhibition is dependent on synaptic activation and glutamate release. A: rod bipolar cells (RBCs) stimulated from a baseline of −100 mV with the same voltage pulse as above, without (gray) and with (black) extracellular application of dl-threo-β-benzyloxyaspartic acid (TBOA). A lack of TBOA-sensitivity confirms that the feedback inhibition attributed to EAAT5 is dependent on synaptic activation. B: RBCs stimulated from a baseline of −60 mV with the same voltage pulse as above immediately following depolarization to −30 mV for 60 s. Lack of TBOA-sensitivity confirms that EAAT5-mediated feedback is dependent on vesicle release. C: summary data representing the difference in charge transfer between two comparitor conditions in individual cells. No stim indicates a cell held at −60 mV without applying a voltage stimulus. Stim, −100 mV baseline, and depolarization indicate experiments depicted in Fig. 2C and here in A and B, respectively. (n = 24 cells). Error bars indicate SE. *P < 0.05.