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. 2020 Jul 31;9:e58619. doi: 10.7554/eLife.58619

Figure 6. Wild-type INX-8 facilitates effects of pod-2(+) overexpression on conditional mutations downstream in the fatty acid synthesis pathway.

Figure 6.

Brood size was measured as the number of viable progeny produced. The balancer mIs11 provided inx-8(+) for all strains listed. (A) Embryonic lethality in emb-8(ts) mutants at 22°C is more severe in heterozygous +/inx-8(rf) than inx-8(+) backgrounds (1; p=0.013)). Additional pod-2(+) expression furnished by Ex[pod-2(+)] in emb-8(ts) significantly rescues embryonic lethality (2; p=1.9 E-05, inx-8[+]; p=1.9 E-05, +/inx-8[rf]; p=0.022, inx-8[rf]). Rescue of emb-8(ts) by Ex[pod-2(+)] is also dependent on inx-8(+) copy number (3; p=5.5 E-03). (B) Expression of Ex[pod-2(+)] in fasn-1(ts) mutants at 20°C reduces brood size (1; p=2.2 E-08, inx-8[+]; p=1.5 E-10, +/inx-8[rf]; p=7.0 E-04, inx-8[rf]). fasn-1(ts); inx-8(+) homozygotes show greater embryonic lethality than fasn-1(ts); (+)/inx-8(rf) heterozygotes in the absence (2; p=0.026) or presence (3; p=0.026) of Ex[pod-2(+)]. Averages indicated with dotted lines. Probability was calculated using Student’s two-tailed t-test. (C) Interpretation of genetic interaction consistent with the results from (A) and (B) in a pathway representation. Relative size of arrows corresponds to expected relative amount of product. Sheath–germline gap junctions composed of INX-8(+) or a mixture of INX-8(+) and INX-8(rf) are positioned as facilitating transfer of malonyl-CoA from soma to germline. Roman numerals correspond to genotypes indicated in (A) and (B). (D) Placement of INX-8 gap junctions downstream of fasn-1 predicts brood size from genotype (vi) would be less than from (v), which is not consistent with the results observed in (B). Figure 6—source data 1 Viable progeny counts for panels (A, B).

Figure 6—source data 1. Brood counts for Figure 6.