FIGURE 7.

ROBO1 participates in neonatal thymus angiogenesis. A, Immunohistochemistry of wild‐type Robo1 ^+/+ mouse neonatal thymus gland showing localization of ROBO1 to the perivascular region of arterioles (results representative tissue from n = 3 animals). Scale bars = 10 μm. B and C, CD31 immunohistochemistry of thymus glands from Robo1 ^+/+ and Robo1 ^−/− neonatal mice with quantification of CD31 vascular density (at least n = 5 sections were analyzed from n = 3 thymus glands per genotype group). Each data point represents a randomly selected field of analysis. Scale bars = 60 μm