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. 2020 May 1;9(9):1053–1067. doi: 10.1002/sctm.19-0438

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© 2020 The Authors. stem cells translational medicine published by Wiley Periodicals Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Identification of adipose tissue‐derived stromal cell (ADSC) pluripotency and homogeneity, and adipogenic induction. A, ADSCs displayed typical fibroblast‐like morphology. Primary cell culturing for 1 day (a), 4 days (b), 7 days (c), and 10 days (d). B, ADSC surface markers were analyzed by flow cytometry. CD29(+) 99.9%, CD90(+) 99.9%, CD31(−) 3.41%, and CD45(−) 1.73%. C, The identification of ADSC multiple differentiation potential. (a) Oil red O staining showing ADSCs differentiating into adipocytes in which the lipid droplets were stained red. (b) Positive alizarin red staining showing calcifying nodules formed after ADSC induction into osteoblasts. (c) Toluidine blue staining showing ADSCs differentiating into chondrocytes. (d) Immunofluorescent staining with NF antibody after ADSC with neurogenic induction for 1 day. (e) DAPI staining of ADSC with neurogenic induction for 1 day. (f) Merged image of (d) and (e)