FIGURE 4.

Long noncoding (lnc)RNA‐Adi is indispensable for adipogenic differentiation of adipose tissue‐derived stromal cells (ADSCs). A, GFP positive cytoplasm image (right) showed that packaged lentivirus with lncRNA‐Adi shRNA sequence successfully transfected into ADSCs. B, ADSCs transfected with lentivirus with lncRNA‐Adi shRNA sequence (lnc‐Adi‐KD) expressed little lncRNA‐Adi during adipogenic induction compared to ADSCs transfected with nothing (ADSCs(BC)) and with lentivirus with nontargeting control sequence (lnc‐Adi‐NC), which verified that the effects of interfering lncRNA‐Adi expression in lnc‐Adi‐KD group ADSCs; n = 3. C, Oil red O staining of ADSCs in BC, NC, and KD groups at day 7. D, Microscopic observation of cell morphology changes in BC, NC, and KD groups at days 0, 3, 5, and 7 after adipogenic induction. E, Microscopic observation of oil red O staining of ADSCs in BC, NC, and KD groups during adipogenic induction at days 0, 3, 5, and 7 under magnifications of 100, 200, and 400. F, Expression level of key adipocytes genes including C/EBPα, PPARγ, aP2, LPL, GLUT4, and leptin in BC, NC, and KD groups during adipogenic induction at days 0, 3, 5, and 7; n = 3