TABLE 1.
Literature comparison of hiPSCs cultured in bioreactor systems
| Reference | Cell lines | Media | Bioreactor size and type | Inoculation and growth platform | Inoculation density (cells/mL) | Feeding strategy | Fold expansion/days |
|---|---|---|---|---|---|---|---|
| Zweigerdt et al 17 | hCBiPSC2, hiPSOCT4eGFP | mTeSR1 | 100 mL, Horizontal‐blade spinner (Integra Biosciences) | Single‐cell inoculation, aggregate growth | 1 × 106 | Media exchange (100%) at 48 h followed by daily media exchange (100%) | 3‐ to 6‐fold/4‐7 d |
| Olmer et al 18 | hCBiPSC2 | mTeSR1 | 100 mL, Pitched blade, computer controlled reactor (DasGip) | Single‐cell inoculation, aggregate growth | 4 × 105 to 5 × 105 | Media exchange (100%) at 72 h followed by daily media exchange (100%) | 5.5‐fold/7 d |
| Abbasalizadeh et al 19 | hiPSC1, hiPSC4 |
DMEM/F12‐CM+ GlutaMAX+bFGF |
100 mL, Horizontal‐blade spinner (Integra Biosciences) | Single‐cell inoculation, aggregate growth | 2 × 105 to 1 × 106 | Media refreshment beginning at 48 h | 8‐fold/7‐10 d |
| Wang et al 20 | BC1, TNC1 | E8 | 100 mL, Glass‐ball spinner flask (CELLSPIN) | Single‐cell inoculation, aggregate growth | 4 × 105 to 5 × 105 | Media exchange (2/3) daily | 3.5‐fold/4 d |
| Elanzew et al 21 | iLB‐C‐31f‐r1 | mTeSR1 or E8 | 50 mL, Tube rotation (BioLevitator) | Single‐cell inoculation, aggregate growth | 7.5 × 104 | Media addition (10 mL) at 24, 48, and 72 h | 5‐fold/4 d |
| Haraguchi et al 22 | 201B7, 253G1 | mTeSR1 |
100 mL, Horizontal‐blade spinner (Integra Biosciences) |
Single‐cell inoculation, aggregate growth | 3 × 105 | Media exchange (100%) daily | 10‐fold/12 d |
| Badenes et al 23 | Gibco CD34+ derived | E8 | 50 mL, Horizontal‐blade spinner flask (StemSpan) | Single‐cell inoculation, polystyrene‐coated microcarrier growth | 2 × 105 to 5 × 105 | Media exchange (80%) at 72 h followed by daily media exchange (80%) | 3.5‐fold/10 d |
| Meng et al 24 | 4YF, 4YA | mTeSR1 | 100 mL, Horizontal‐blade spinner (NDS) | Preformed aggregate inoculation, aggregate growth | 2 × 104 to 8 × 104 | Media exchange (100%) on days when culture pH measured below 7.0 | 11‐ to 13‐fold/5 d |
| Kwok et al 25 | AFiPS, FSiPS |
mTeSR1 or StemMACs iPSC‐Brew |
1. 100 mL, Horizontal‐blade spinner 2. 1 L, Horizontal‐blade, single‐use bioreactor |
Single‐cell inoculation, aggregate growth | 2 × 105 |
1. Media addition (20 mL) at 48 and 72 h followed by daily media exchange (100%) 2. Media addition (250 mL) at 48 and 72 h followed by daily media exchange (100%) |
1. 16‐fold /7 d 2. 10‐fold /7 d |
| Abecasis et al 26 | ChiPSC4 | Cellartis DEF‐CS Xeno‐Free Culture Medium | 200 mL, Trapezoid paddle, computer controlled reactor (DasGip) | Single‐cell inoculation, aggregate growth | 2.5 × 105 to 5 × 105 | Perfusion system (initiated at 24 h) simulating media renewal frequency established in static monolayer culture (dilution rate = 1.3 d−1) | 19‐fold/4 d |
| This study | 4YA | mTeSR1 | 100 mL, Vertical‐wheel, single‐use spinner (PBS Biotech) | Preformed aggregate inoculation, aggregate growth | 2 × 104 | Media exchange (50 mL) at 96 h | 32‐fold/6 d |
Note: Referenced data include cell lines, media, bioreactor size and type, inoculation and growth platform, inoculation density, feeding strategy, and fold expansion/days.
Abbreviation: DMEM, Dulbecco's modified Eagle medium; hiPSCs, human induced pluripotent stem cells.